MicroRNAs (miRNAs) are found in eukaryotes, which has the function of regulation and control of a class of endogenous non-coding RNA, its size is about 20 ~ 25 nucleotides long. Mature miRNAs identified target mRNA by means of complementary base pairing, and depending on the degree of complementary guidance silence complex target mRNA degradation or deter a translation of the target mRNA.
Transcription factors is a group of 5 ` end with gene upstream specificity combined with a specific sequence, so as to ensure the strength of purpose gene in a particular during a specific time and space expression of the protein molecule. Transcription factor binding site (TFBS) is a transcription factor to regulate gene expression, and genetic template strand area
Application of miRNA
The role of micrornas in disease treatment: 1. Cholesterol homeostasis of mirnas can be adjusted. 2. Adjust the switch the miRNA - 132 blood vessel growth. 3. The miRNA - 193 - a has tumor suppressor function.
Application of TF
At present, the Artificial Transcription Factor (ATF) building has been used in the study of biological function of Transcription factors play an important role. ATF is refers to the combination of different DNA structure and effect structure domains together, artificially build has the new sequence specificity and the effect of the transcription factor, in basic research, drug design, and gene therapy, and other fields has been widely studied